ΤΡΙΑΝΤΑΦΥΛΛΟΣ ΣΤΥΛΙΑΝΟΠΟΥΛΟΣ
ΣΤΥΛΙΑΝΟΠΟΥΛΟΣ ΤΡΙΑΝΤΑΦΥΛΛΟΣ
STYLIANOPOULOS TRIANTAFYLLOS
...
ΑΝΑΠΛΗΡΩΤΗΣ/ΡΙΑ ΚΑΘΗΓΗΤΗΣ/ΡΙΑ
Τμήμα Μηχανικών Μηχανολογίας και Κατασκευαστικής
Green Park
Λεωφ. Αγλαντζιας 91
507
+357 2289 2238
+357 2289 5081
www.ucy.ac.cy/cancer_biophysics/en

    Προσωπικό Προφίλ

    Diploma in Chemical Engineering, 2003
    National Technical University of Athens, Greece
     
    PhD in Chemical Engineering, 2008
    University of Minnesota, USA
     
    Postdoctoral training, Department of Radiation Oncology
    Massachusetts General Hospital / Harvard Medical School, USA
      
    Triantafyllos received a Diploma in Chemical Engineering from National Technical University of Athens, Greece (GPA: 9.34/10) in 2003. The same year he joined the Chemical Engineering graduate program at the University of Minnesota, USA to pursue his doctoral studies. He worked with Professor Victor Barocas on modeling the mechanical response of native and bioartificial tissues. During his doctoral studies, he was elected twice top doctoral student of the department. After the completion of his PhD., in May 2008, Triantafyllos joined Dr. Rakesh Jain's lab in the department of Radiation Oncology at Harvard Medical School and Massachusetts General Hospital, USA for his prostodoctoral training. In Dr. Jain's lab he performed research in a mixture of experimental and computational problems for optimizing the delivery of molecular and nanoscale medicine to solid tumors. In September 2010, he joined the faculty of the Mechanical and Manufacturing Engineering department at the University of Cyprus.
     
    Triantafyllos has co-authored over 105 publications in peer-review journals. His publication record includes five articles in Nature journals (Nature Nanotechnology, Nature Medicine, Nature Communications and two articles in Nature Reviews Clinical Oncology), eight articles in PNAS, two in Cancer Research as well as articles in Clinical Cancer Research and ACS Nano. h-index:34, > 8000 citations (Web-of-Science) | h-index:40, > 10600 citations (GoogleScholar).
     
    He has received 2 highly selective research grant by the European Research Council (ERC Starting Grant ReEngineeringCancer, 2014-2018, 1.44M euros and ERC Consolidator Grant Immuno-Predictor, 2020-2025, 2.0M euros) as well as the prestigious Y. C. Fung Early Carrer award by the American Society of Mechanical Engineers in 2016 (https://www.asme.org/about-asme/participate/honors-awards/achievement-awards/y-c-fung-young-investigator-award). Triantafyllos was also the recipient of the 2014 Most Cited Paper Award by the Biomedical Engineering Society (BMES) of the USA in 2014 and the New Researcher Award by the Research Promotion Foundation of Cyprus the same year.   
     
    The last 6 years, Triantafyllos has received as a Prinicipal Investigator ~ 5.5 Millions in Research funding, including 7 grants from FP7 and Horizon2020 and 2 grants from Research Promotion Foundation of Cyprus. 
     
    Triantafyllos is a member of the American Society of Mechanical Engineers, the Biomedical Engineering Society of the USA, the European Foundation of Clinical Nanomedicine, the European Society of Biomechanics and the Greek Association of Computational Mechanics.

    The major focus of my current research is on the biomechanics and drug delivery of solid tumors. I use experimental methods in mice bearing tumors and in vitro tumor models as well as mathematical modelling to study the evolution of mechanical forces in tumors and how these forces correlate with tumors' patho-physiology and delivery of chemotherapy, nanomedicine and immunotherapy. Furthermore, I investigate strategies to optimise tumor blood vessel functionality in order to improve systemic delivery of cancer therapeutics by modifying the mechanical tumor micro-environment.

     

    Current Funding ID:
    - Research Promotion Foundation of Cyprus, INFRASTRUCTURES/1216/0052, Title: "CancerNanoMED:  Center for Preclinical Evaluation and Optimization of Cancer Nanomedicines".  € 995,812. December 2018 - November 2022.

    - European Research Council, Proof of Concept Grant, (ERC-2018-PoC-838414, CancerFingerPrints), Title: “Identification of nano-mechanical fingerprints as a biomarker for cancer treatment prognosis”. €150,000, May 2019-October 2020.

    - Horizon 2020, Marie Skłodowska-Curie Individual Fellowship, (MSCA-IF-2017-793569 NanoFEITH). Title: "Nanoparticles for fluorescence-enhanced imaging and therapy of breast  cancer ".  €151,649. June  2018 - May 2020.

    - Horizon 2020, Marie Skłodowska-Curie Individual Fellowship, (WF-01-2018-867455 Widening Fellowships, ImmunoMECH), Title: " High-performance biomechanical model of combined immunotherapy and antiangiogenic cancer treatment".  €145, 941. June 2019 - May 2020.

    - Research Promotion Foundation of Cyprus, POST-DOC/0718/0084, Title: “Optimizing immunotherapy in triple-negative breast cancer by normalizing the tumor microenvironment”. €159,924. Expected duration: January 2020-December 2022.

    PAST

    - European Research Council (ERC) Starting Grant (ERC-2013-StG-336839 ReEngineeringCancer).  Title: "Re-engineering the tumor microenvironment to alleviate mechanical stresses and improve chemotherapy".  € 1,440,360. January 2014 - December 2018.   

    - Horizon 2020, Marie Skłodowska-Curie Individual Fellowship, (MSCA-IF-2014-657139 STROMAMECH). Grant Title: "Targeting stromal cells to modify tumor mechanical microenvironment  and optimize drug delivery".  €151,649. May 2015 - April 2017.

     -  Horizon 2020, Marie Skłodowska-Curie Individual Fellowship, (MSCA-IF-2014-658769-MYO-DESMOPLASIA). Grant Title: "Modulating the behaviour of cancer myofibroblasts to control tumour desmoplasia".  €163,649. June 2015 - May 2017.

    -  Young Investigator Award, Research Promotion Foundation of Cyprus, €35,000. June 2015- May 2017.

    -  FP7 Marie-Curie International Reintegration Grant (PIRG08-GA-2010-276894 Cancer Nanomedicine).    Grant Title: "Optimizing the Delivery of Nanomedicine to Solid Tumors". €100,000. August 2011- July  2015.

     (98 articles, h-index=31, >6440 citations Web-of-Science | h-index=36, >8500 citations Google Scholar

     * denotes corresponding authorship

     2019

    98. Martin J.D., H. Cabral, T. Stylianopoulos*, and R.K. Jain. Improving cancer immunotherapy using nanomedicine: progress, opportunities and challenges. Nature Reviews Clinical Oncology (Accepted).

    97.  Panagi M. , C. Voutouri, F. Mpekris, P. Papageorgis, M.R. Martin, J.D. Martin, C. Polydorou, M. Kojima, G. Ishii, K. Kataoka and H. Cabral and T.  Stylianopoulos*. TGF-β inhibition combined with cytotoxic nanomedicine normalizes triple negative breast cancer microenvironment towards anti-tumor immunity. Theranostics (Accepted)

    96. Katsamba I., P. Evangelidis and T. Stylianopoulos*. Biomechanical modelling of spinal tumor anisotropic growth. (Under review).

    95. Kalli M., C. Voutouri, A. Minia, V. Pliaka, C. Fotis, L.G. Alexopoulos, and T. Stylianopoulos*. Mechanical Compression inhibits the growth of brain cancer cell spheroids and regulates cell migration through MEK1/Erk1 pathway and GDF15 expression. (Accepted in Frontiers in Oncology).

    94. Gkretsi V., A. Stylianou, M. Kalli, M. Louca, C. Voutouri, and T. Stylianopoulos*.  Growth Differentiation Factor-15 (GDF-15) inhibits cell invasion in breast cancer cells in vitro by suppressing the expression of focal adhesion proteins. (Under review).

    93. Kontomaris S. V., A. Stylianou, K.S. Nikita, A. Malamou, T. Stylianopoulos*. A simplified approach for the determination of fitting constants in Oliver – Pharr method regarding biological samples. Phys. Biol [DOI: 10.1088/1478-3975/ab252e].

    92. Stylianou A., V. Gkretsi, M. Louca, L.C. Zacharia and T. Stylianopoulos*. Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts. Journal of the Royal Society Interface [DOI: 10.1098/rsif.2019.0226].

    91. Gkretsi V., M. Kalli, C. Efstathiades, P. Papageorgis, V. Papanikolaou, L.C. Zacharia, A. Tsezou, E. Athanassiou, T. Stylianopoulos*, Depletion of Ras Suppressor-1 (RSU-1) promotes cell invasion of breast cancer cells through a compensatory upregulation of a truncated isoform. Scientific Reports [DOI: 10.1038/s41598-019-46575-0]

    90. Louca M., V. Gkretsi, and T. Stylianopoulos*. Coordinated Expression of Ras Suppressor 1 (RSU-1) and Growth Differentiation Factor 15 (GDF15) Affects Glioma Cell Invasion. Cancers  [DOI: 10.3390/cancers11081159].

    89. Martin J.D., M. Panagi, C. Wang, T.T. Khan, M.R. Martin, C. Voutouri, K. Toh, P. Papageorgis, F. Mpekris, C. Polydorou, G. Ishii, S. Takahashi, N. Gotohda, T. Suzuki, M.E. Wilhelm, V.A. Melo, S. Quader, J. Norimatsu, R.M. Lanning, M. Kojima, M.D. Stuber, T. Stylianopoulos, H. Cabral, and K. Kataoka and H. Cabra. The antiemetic dexamethasone increases nanocarrier delivery by normalizing the tumor microenvironment. ACS Nano [DOI: 10.1021/acsnano.8b07865].

    88. Louca M., A. Stylianou, A. Minia, V. Pliaka, L.G. Alexopoulos V. Gkretsi and T. Stylianopoulos*. Ras suppressor-1 (RSU-1) promotes cell invasion in aggressive glioma cells and inhibits it in non-aggressive cells through STAT6 phospho-regulation. Scientific Reports [DOI: 10.1038/s41598-019-44200-8]

    87. Mascheroni P., C.L. Juan, M. Kalli, T. Stylianopoulos, M.H. Michael and H. Hatzikirou. On the impact of chemo-mechanically induced phenotypic transitions in gliomas. Cancers  [DOI: 10.3390/cancers11050716].

    86. Demetriadou C., D. Pavlou, F. Mpekris, C. Achilleos, T. Stylianopoulos, A. Zaravinos, P. Papageorgis and A Kirmizis, Naa40 contributes to colorectal cancer growth by controlling PRMT5 expression. Cell Death & Disease [DOI: 10.1038/s41419-019-1487-3].

    2018

    85. Voutouri C., N.D. Kirkpatrick, E. Chung, F. Mpekris, J.W. Baish, L.L. Munn, D. Fukumura, T. Stylianopoulos* and R.K. Jain. Dynamics of vessel cooption in brain tumors revealed by integrative experimental and mathematical modeling studies. PNAS  [DOI: 10.1073/pnas.1818322116].

    84. Zhao Y., J. Cao, A. Melamed, M. Worley, A. Gockley, D. Jones, H.T. Nia, Y. Zhang, T. Stylianopoulos, A.S. Kumar, F. Mpekris, M. Datta, Y. Sun, L. Wu, X. Gao, O. Yeku, M. del Carmen, D.R. Spriggs, R.K. Jain, and L. Xu. Losartan treatment enhances chemotherapy efficacy and reduces ascites in ovarian cancer models by normalizing the tumor stroma. PNAS [DOI: 10.1073/pnas.1818357116].

    83. Kalli M., F. Mpekris, K.C. Wong, M. Panagi, S. Ozturk, S. Thiagalingam, T. Stylianopoulos and P. Papageorgis. Activin A signaling regulates IL13Rα2 expression to promote breast cancer metastasis. Frontiers in Oncology (Accepted).

    82. Gkretsi, V., Louca, M., Stylianou, A., George Minadakis, G., Spyrou, G.M and Stylianopoulos*, T. Inhibition of breast cancer cell invasion by Ras suppressor-1 (RSU-1) silencing is reversed by Growth Differentiation Factor-15 (GDF-15). Journal of Molecular Sciences, 20 (1) [DOI: 10.3390/ijms20010163].

    81. Kalli M., A. Minia, V. Pliaka, C. Fotis, L.G. Alexopoulos, T. Stylianopoulos*, Solid stress-induced migration is mediated by GDF15 through Akt pathway activation in pancreatic cancer cells. Scientific Reports [DOI: 10.1038/s41598-018-37425-6].

    80. Stylianou A., M. Lekka and T. Stylianopoulos*. AFM Assessing of Nanomechanical FingerPrints for Cancer Early Diagnosis and Classification: from single cell to tissue level. Nanoscale [DOI: 10.1039/C8NR06146G].

    79. Angeli S., K. E. Emblem, P. D. Tonnessen and T. Stylianopoulos*. Towards patient-specific modeling of brain tumor growth and formation of secondary nodes guided by DTI-MRI. NeuroImage: Clinical [DOI: 10.1016/j.nicl.2018.08.032].

    78. Vavourakis V., T. Stylianopoulos, P.A. Wijeratne. In-silico Dynamic Analysis of Cytotoxic Drug Administration to Solid Tumours: Effect of Binding Affinity and Vessel Permeability. PLoS Computational Biology 14(10):e1006460 [DOI: 10.1371/journal.pcbi.1006460].

    77. Kontomaris S.V., A. Stylianou, A. Malamou and T. Stylianopoulos*. A simplified AFM-based model for the calculation of the Young's modulus of cylindrical and spherical shaped samples. Materials Research Express [DOI: 10.1088/2053-1591/aad2c9]

    76. Kitiri E.N., C.K. Varnava, C.S. Patrickios, Voutouri, T. Stylianopoulos, M. Gradzielski and I. Hoffmann. Double-networks Based on Interconnected Amphiphilic “In-out” Star First Polymer Conetworks Prepared by RAFT Polymerization. Journal of Polymer Science, Part A: Polymer Chemistry. [DOI: doi.org/10.1002/pola.29176](Accepted)

    75. Stylianou A., V. Gkretsi, and T. Stylianopoulos*. Atomic Force Microscopy Nano-Characterization of 3D Collagen Gels with Tunable Stiffness. MethodsX  5:503-513 [DOI: 10.1016/j.mex.2018.05.009]

    74. Papaparaskeva G., C. Voutouri, V. Gkretsi, M. Achilleos, T. Stylianopoulos and T. Krasia-Christoforou. Tuning the Mechanical Properties of BIEE-crosslinked Semi-Interpenetrating, Double-Hydrophilic Hydrogels. Macromolecular Materials and Engineering [DOI: 10.1002/mame201700643].

    73. Gkretsi V., and T. Stylianopoulos*. Cell adhesion and matrix stiffness: coordinating cancer cell invasion and metastasis. Frontiers in Oncology  8:145 [DOI: 10.3389/fonc.2018.00145].

    72. Mpekris F., C. Voutouri, P. Papageorgis and T. Stylianopoulos*. Stress alleviation strategy in cancer treatment: Insights from a mathematical model.  Z Angew Math Mech. 2018;1–12 [DOI: 10.1002/zamm.201700270].

    71. Stylianopoulos* T., L.L. Munn, and R.K. Jain. Reengineering the physical microenvironment of tumors to improve drug delivery and efficacy: From math modeling to bench to bedside. Trends in Cancer  4(4):292-319 [DOI: 10.1016/j.trecan.2018.02.005].

    70. Voutouri C. and T. Stylianopoulos*. Accumulation of mechanical forces in tumors is related to hyaluronan content and tissue stiffness. PLoS One 13(3): e0193801 [DOI: 10.1371/journal.pone.0193801].

    69. Kalli M. and T. Stylianopoulos*. Defining the role of solid stress and matrix stiffness in cancer cell proliferation and metastasis. Frontiers in Oncology  8:55 [DOI: 10.3389/fonc.2018.00055].

    68. Stylianou A., V. Gkretsi and T. Stylianopoulos*. Transforming Growth Factor-β modulates Pancreatic Cancer Associated Fibroblasts cell shape, stiffness and invasion. BBA  1862(7):1537-1546 [DOI: 10.1016/j.bbagen.2018.02.009].

    67. Kalli M., P. Papageorgis, V. Gkretsi and T. Stylianopoulos*. Solid stress facilitates fibroblasts activation to promote pancreatic cancer cell migration. Annals of Biomedical Engineering. 46(5):657-669 [DOI: 10.1007/s10439-018-1997-7].

    66. Zacharia LC, T. Stylianopoulos, and V. Gkretsi. Ras Suppressor-1 (RSU-1) in cancer cell metastasis: friend or foe? Critical Reviews in Oncogenesis. 22(3-4):249-253 [DOI: 10.1615/CritRevOncog.2018024231].

    2017

    65. Stylianou A., Gkretsi V., Patrickios C.S., Stylianopoulos* T. Exploring the Nano-Surface of Collagenous and Other Fibrotic Tissues with AFM. In: Rittié L. (eds) Fibrosis. Methods in Molecular Biology, vol 1627. Humana Press, New York, NY [DOI: 0.1007/978-1-4939-7113-8_29]

    64. P.A. Wijeratne, J.H. Hipwell, D.J. Hawkes, T. Stylianopoulos and V. Vavourakis. Multiscale biphasic modelling of peritumoural collagen microstructure: the effect of tumor growth on permeability and fluid flow. PlosOne 12(9): e0184511. [DOI: 10.1371/journal.pone.0184511]

    63. Gkretsi V., L.C. Zacharia, and T. Stylianopoulos*. Targeting inflammation to improve tumor drug delivery. Trends in Cancer 3(9): 621-630. [DOI: 10.1016/j.trecan.2017.07.006]

    62. Mpekris F., P. Papageorgis, C. Polydorou, C. Voutouri, M. Kalli, A.P. Pirentis and T. Stylianopoulos*. Sonic-hedgehog pathway inhibition normalizes desmoplastic tumor microenvironment to improve chemo- and nanotherapy. Journal of Controlled Release 261(10): 105-112. [DOI: 10.1016/j.jconrel.2017.06.022]

    61. Papageorgis P., C. Polydorou, F. Mpekris, C. Voutouri, E. Agathokleous, C. P. Kapnissi-Christodoulou, and T. Stylianopoulos*. Tranilast-induced stress alleviation in solid tumors improves the efficacy of chemo- and nanotherapeutics in a size-independent manner. Scientific Reports 7:46140. [DOI: 10.1038/srep46140]

    60. Gkretsi V., A. Stylianou, M. Louca, and T. Stylianopoulos*. Identification of Ras suppressor-1 (RSU-1) as a potential breast cancer metastasis biomarker using a three-dimensional in vitro approach. Oncotarget 8(16):27364-27379. [DOI: 10.18632/oncotarget.16062]

    59. Angeli S., and T. Stylianopoulos*. Experimental measurements and mathematical modeling towards quantification of brain swelling stress. Journal of Biomechanics 56:42-47. [DOI: 10.1016/j.jbiomech.2017.02.028]

    58. Polydorou C., F. Mpekris, P. Papageorgis, C. Voutouri, and T. Stylianopoulos*. Pirfenidone normalizes the tumor microenvironment to improve chemotherapy. Oncotarget 8(15):24506-24517.[DOI: 10.18632/oncotarget.15534]

    57. Gkretsi V., A. Stylianou, and T. Stylianopoulos*. Vasodilator-Stimulated Phosphoprotein (VASP) depletion from breast cancer MDA-MB-231 cells inhibits tumor spheroid invasion through downregulation of Migfilin, β-catenin and urokinase-plasminogen activator (uPa). Experimental Cell Research 352(2): 281-292. [DOI: 10.1016/j.yexcr.2017.02.019]

    56. Mpekris F., J.W. Baish, T. Stylianopoulos*, and R.K. Jain. Role of vascular normalization in benefit from metronomic chemotherapy. Proceedings of the National Academy of Sciences USA 114(8): 1994-1999. [DOI: 10.1073/pnas.1700340114]

    55. Ambrosi D., S. Pezzuto, D. Riccobelli, T. Stylianopoulos, and P. Ciarletta. Solid tumors are poroelastic solid with chemo-mechanical feedback on growth. Journal of Elasticity 129(1-2):107-124 [DOI: 10.1007/s10659-016-9619-9]

    54. Vavourakis V., P.A. Wijeratne, R. Shipley, M. Loizidou, T. Stylianopoulos, and D.J. Hawkes. A validated multiscale in-silico model for mechano-sensitive tumour angiogenesis and growth. PLoS Computational Biology 13(1): e1005259. [DOI: 10.1371/journal.pcbi.1005259]

    53. Stylianopoulos* T. The solid mechanics of cancer and strategies for improved therapy. Journal of Biomechanical Engineering 139(2): 021004. [DOI: 10.1115/1.4034991]

    52. Kitiri E., C. Patrickios, C. Voutouri, T. Stylianopoulos, I. Hoffmann, R. Schweins, and M. Gradzielski. Synthesis and characterization of double-networks based on pH-responsive, amphiphilic "core-first" star first polymer conetworks prepared by sequential RAFT polymerization. Polymer Chemistry 8(1): 245-259. [DOI: 10.1039/C6PY01340F]

    2016

    51. Alfonso J.C.L., A. Köhn-Luque, T. Stylianopoulos, F. Feuerhake, A. Deutsch, and H. Hatzikirou. Why one-size-fits-all vaso-modulatory interventions fail to control glioma invasion: in silico sights. Scientific Reports 6: 37283. [DOI: 10.1038/srep37283]

    50. Voutouri C., C. Polydorou, P. Papageorgis, V. Gkretsi, and T. Stylianopoulos*. Hyaluronan-derived swelling of solid tumours, the contribution of collagen and cancer cells, and implications for cancer therapy. Neoplasia 18(12): 732-741. [DOI: 10.1016/j.neo.2016.10.001]

    49. Wijeratne P.A., V. Vavourakis, J.H. Hipwell, C. Voutouri, P. Papageorgis, T. Stylianopoulos, A. Evans, and D.J. Hawkes. Multiscale modelling of solid tumour growth: the effect of collagen micromechanics. Biomechanics and Modeling in Mechanobiology 15(5): 1079-1090. [DOI: 10.1007/s10237-015-0745-2]

    48. Achilleos M., F. Mpekris, T. Stylianopoulos, and T. Krasia-Christoforou. Structurally-defined semi-interpenetrating amphiphilic polymer networks with tunable and predictable mechanical response. RSC Advances 6(49): 43278-43283. [DOI: 10.1039/C6RA07376J]

    47. Angeli S., and T. Stylianopoulos*. Biphasic modeling of brain tumor biomechanics and response to radiation treatment. Journal of Biomechanics49(9): 1524-1531. [DOI: 10.1016/j.jbiomech.2016.03.029]

    46. Stylianou A., and T. Stylianopoulos*. Atomic force microscopy probing of cancer cells and tumor microenvironment components. BioNanoScience6(1): 33-46. [DOI: 10.1007/s12668-015-0187-4]

    45. Stylianopoulos* T. Intelligent drug delivery systems for the treatment of solid tumors. European Journal of Nanomedicine 8(1): 9-16. [DOI: 10.1515/ejnm-2015-0041]

    2015

    44. Savva I., E. Evaggelou, G. Papaparaskeva, T. Leontiou, T. Stylianopoulos, F. Mpekris, K. Stylianou, and T. Krasia-Christoforou. Alignment of electrospun polymer fibers using a concave collector. RSC Advances 5(126): 104400-104407. [DOI: 10.1039/C5RA20429A]

    43. Gkretsi V., A. Stylianou, P. Papageorgis, C. Polydorou, and T. Stylianopoulos* T. Remodeling components of the tumor microenvironment to enhance cancer therapy. Frontiers in Oncology 5: 214. [DOI: 10.3389/fonc.2015.00214]

    42. Kepola E.J, E. Loizou, C.S. Patrickios, E. Leontidis, C. Voutouri, T. Stylianopoulos, R. Schweins, M. Gradzielski, C. Krumm, J.C. Tiller, M. Kushnir, and C. Wesdemiotis. Amphiphilic polymer conetworks based on end-linked "core-first" star block copolymers: structure formation with long-range order. ACS Macro Letters 4(9): 1163-1168 (2015) [DOI: 10.1021/acsmacrolett.5b00608]

    41. Stylianopoulos* T., and R.K. Jain. Design considerations for nanotherapeutics in oncology. Nanomedicine: Nanotechnology, Biology and Medicine 11(8): 1893-1907. [DOI: 10.1016/j.nano.2015.07.015]

    40. Papaphilippou P.Ch., I. Vyrides, F. Mpekris, T. Stylianopoulos, C.A. Papatryphonos, C.R. Theocharis, and T. Krasia-Christoforou. Evaluation of novel, cationic electrospun microfibrous membranes as adsorbents in bacteria removal. RSC Advances 5(83): 67617-67629. [DOI: 10.1039/C5RA11406C]

    39. Duda D.G., R.S. Heist, D.V. Sahani, T. Stylianopoulos, J.A. Engelman, and R.K. Jain. Reply to Ciccolini et al.: Using mathematical modeling to predict response to antiangiogenic therapy in cancer patients. Proceedings of the National Academy of Sciences USA 112(27): E3454. [DOI: 10.1073/pnas.1507225112]

    38. Mpekris F., S. Angeli, A.P. Pirentis, and T. Stylianopoulos*. Stress-mediated progression of solid tumors: effect of mechanical stress on tissue oxygenation, cancer cell proliferation and drug delivery. Biomechanics and Modeling in Mechanobiology 14(6): 1391-1402. [DOI: 10.1007/s10237-015-0682-0]

    37. Pirentis A.P., C. Polydorou, P. Papageorgis, C. Voutouri, F. Mpekris, and T. Stylianopoulos*. Remodelling of extracellular matrix due to solid stress accumulation during tumour growth. Connective Tissue Research 56(5): 345-354. [DOI: 10.3109/03008207.2015.1047929]

    36. Stylianopoulos* T., E.-A. Economides, J.W. Baish, D. Fukumura, and R.K. Jain. Towards optimal design of cancer nanomedicines: multi-stage nanoparticles for the treatment of solid tumors. Annals of Biomedical Engineering 43(9): 2291-2300. [DOI: 10.1007/s10439-015-1276-9]

    35. Mpekris F., M. Achilleos, E. Vasile, E. Vasile, T. Krasia-Christoforou, and T. Stylianopoulos*. Mechanical properties of structurally-defined magnetoactive polymer (co)networks. RSC Advances 5(26): 20011-20019. [DOI: 10.1039/C4RA16260A]

    34. Papageorgis P., and T. Stylianopoulos*. Role of TGFβ in regulation of the tumor microenvironment and drug delivery. International Journal of Oncology 46(3): 933-943. [DOI: 10.3892/ijo.2015.2816]

    2014

    33. Voutouri C., and T. Stylianopoulos*. Evolution of osmotic pressure in solid tumors. Journal of Biomechanics 47(14): 3441-3447. [DOI:10.1016/j.jbiomech.2014.09.019]

    32. Voutouri C., F. Mpekris, P. Papageorgis, A.D. Odysseos, and T. Stylianopoulos*. The role of constitutive behavior and tumor-host mechanical interactions in the state of stress and growth of solid tumors. PLoS One 9(8): e104717. [DOI: 10.1371/journal.pone.0104717]

    31. Chauhan V.P., Y. Boucher, C.R. Ferrone, S. Roberge, J.D. Martin, T. Stylianopoulos, N. Bardeesy, R.A. DePinho, T.P. Padera, L.L. Munn, and R.K. Jain. Compression of pancreatic tumor blood vessels by hyaluronan is caused by solid stress and not interstitial fluid pressure. Cancer Cell 26(1): 14-15. [DOI: 10.1016/j.ccr.2014.06.003]

    30. Jain R.K., J.D. Martin, and T. Stylianopoulos*. The role of mechanical forces in tumor progression and therapy. Annual Reviews of Biomedical Engineering 16: 321-346. [DOI: 10.1146/annurev-bioeng-071813-105259]

    29. Achilleos A., C. Loizidis, M. Hadjiandreou, T. Stylianopoulos, and G. Mitsis. Multiprocessing linear dynamics modeling for personalized tumor growth prediction. Annals of Biomedical Engineering 42(5): 1095-1111. [DOI: 10.1007/s10439-014-0975-y]

    2013

    28. Stylianopoulos* T., and R.K. Jain. Combining two strategies to improve perfusion and drug delivery in solid tumors. Proceedings of the National Academy of Sciences USA 110(46): 18632-18637. [DOI: 10.1073/pnas.1318415110]

    27. Huang Y., T. Stylianopoulos, D. Duda, D. Fukumura, and R.K. Jain. Benefits of vascular normalization are dose and time dependent. Cancer Research 73(23): 7144-7146. [DOI: 10.1158/0008-5472.CAN-13-1989]

    26. Chauhan V.P., J.D. Martin, H. Liu, D.A. Lacorre, S.R. Jain, S.V. Kozin, T. Stylianopoulos, A.S. Mousa, X. Han, P. Adstamongkonkul, Z. Popovic, P. Huang, M.G. Bawendi, Y. Boucher and R.K. Jain. Angiotensin inhibition enhances drug delivery and potentiates chemotherapy by decompressing tumor blood vessels. Nature Communications 4: 2516. [DOI: 10.1038/ncomms3516]

    25. Stylianopoulos* T., J.D. Martin, M. Snuderl, F. Mpekris, S. Jain, and R.K. Jain. Coevolution of solid stress and interstitial fluid pressure in tumor during progression: Implications for vascular collapse. Cancer Research 73(13): 3833-3841. [DOI: 10.1158/0008-5472.CAN-12-4521]

    24. Stylianopoulos* T. EPR-effect: utilizing size-dependent nanoparticle delivery to solid tumors. Therapeutic delivery 4.4: 421-423. [DOI: 10.4155/tde.13.8]

    23. Stylianopoulos* T., K. Soteriou, D. Fukumura, and R.K. Jain. Cationic nanoparticles have superior transvascular flux in solid tumors: Insights from a mathematical model. Annals of Biomedical Engineering 41(1): 68-77. [DOI: 10.1007/s10439-012-0630-4]

      

    2012

    22. Stylianopoulos* T., M. Kokonou, S. Michael, A. Tryfonos, C. Rebholz, A.D. Odysseos, and D. Doumanidis. Tensile mechanical properties and hydraulic permeabilities of electrospun cellulose acetate fiber meshes. Journal of Biomedical Materials Research - Part B Applied Biomaterials 100(8): 2222-2230. [DOI: 10.1002/jbm.b.32791]

    21. Stylianopoulos T., J.D. Martin, V.P. Chauhan, S.R. Jain, B. Diop-Frimpong, B. Smith, C.R. Ferrone, F. Hornicek, Y. Boucher, L.L. Munn, and R.K. Jain. Causes, consequences, and remedies for growth-induced solid stress in murine and human tumors. Proceedings of the National Academy of Sciences USA 109(38): 15101-15108. [DOI: 10.1073/pnas.1213353109]

    20. Chauhan V.P., T. Stylianopoulos, J.D. Martin, Z. Popovic, W.S. Kamoun, M.G. Bawendi, D. Fukumura, and R.K. Jain. Normalization of tumour blood vessels improves the delivery of nanomedicines in a size-dependent manner. Nature Nanotechnology 7: 383-388. [DOI: 10.1038/nnano.2012.45]

    19. Stylianopoulos T., C. Wong, M.G. Bawendi, R.K. Jain, and D. Fukumura. Multistage nanoparticles for improved delivery into tumor tissue. Methods in Enzymology 508: 109-130. [DOI: 10.1016/B978-0-12-391860-4.00006-9]

    PI's pre-CBL publications

    2011

    18. Chauhan V.P., T. Stylianopoulos, Y. Boucher, and R.K. Jain. Delivering of molecular and nanoscale medicine to tumors: transport barriers and strategies. Annual Reviews of Chemical and Biomolecular Engineering 2: 281-298. [DOI: 10.1146/annurev-chembioeng-061010-114300]

    17. Wong C.R., T. Stylianopoulos, J. Cui, J. Martin, V.P. Chauhan, W. Jiang, Z. Popovic, R.K. Jain, M.G. Bawendi, and D. Fukumura. Multistage nanoparticle delivery system for deep penetration into tumor tissue. Proceedings of the National Academy of Sciences USA 108(6): 2426-2431. [DOI: 10.1073/pnas.1018382108]

    16. Baish J.W., T. Stylianopoulos, R.M. Lanning, W. Kamoun, L.L. Munn, D. Fukumura, and R.K. Jain. Scaling rules for diffusive drug delivery in tumor and normal tissues. Proceedings of the National Academy of Sciences USA 108(5): 1799-1803. [DOI: 10.1073/pnas.1018154108]

    2010

    15. Stylianopoulos T., B. Diop-Frimpong, L.L. Munn, and R.K. Jain. Diffusion anisotropy in collagen gels and tumors: the effect of fiber network orientation. Biophysical Journal 99(10): 3119-3128. [DOI: 10.1016/j.bpj.2010.08.065]

    14. Jain R.K., and T. Stylianopoulos. Delivering nanomedicine to solid tumors. Nature Reviews Clinical Oncology 7(11): 653-664. [DOI: 10.1038/nrclinonc.2010.139]

    13. Stylianopoulos T., M.Z. Poh, N. Insin, L.L. Munn, D. Fukumura, M. Bawendi, and R.K. Jain. Diffusion of particles in the extracellular matrix: effect of repulsive electrostatic interactions. Biophysical Journal 99(5): 1342-1349. [DOI: 10.1016/j.bpj.2010.06.016]

    2009

    12. Vakoc V.J., R.M. Lanning, J.A. Tyrrell, T.P. Padera, L. Bartlett, T. Stylianopoulos, L.L. Munn, G.J. Tearney, D. Fukumura, R.K. Jain, and B.E. Bouma. Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging. Nature Medicine 15(10): 1219-1223. [DOI: 10.1038/nm.1971]

    11. Mok W., T. Stylianopoulos, Y. Boucher, and R.K. Jain. Mathematical modeling of herpes simplex virus distribution in solid tumors: implications for cancer gene therapy. Clinical Cancer Research 15(7): 2352-2360. [DOI: 10.1158/1078-0432.CCR-08-2082]

    10. Sander E.A., T. Stylianopoulos, R.T. Tranquillo, and V.H. Barocas. Image-based multi-scale modeling predicts tissue-level and network-level fiber reorganization in stretched cell-compacted collagen gels. Proceedings of the National Academy of Sciences USA 106(42): 17675-17680. [DOI: 10.1073/pnas.0903716106]

    9.  Sander E.A., T. Stylianopoulos, R.T. Tranquillo, and V.H. Barocas. Image-based biomechanics of collagen-based tissue equivalents. IEEE Engineering in Medicine and Biology 28(3): 10-18. [DOI: 10.1109/MEMB.2009.932486]

    8.  Luo X., T. Stylianopoulos,V.H. Barocas, and M.S. Shephard. Multiscale computation for bioartificial soft tissues with complex geometries. Journal of Engineering with Computers 25(1): 87-95. [DOI: 10.1007/s00366-008-0111-4]

    2008

    7.  Stylianopoulos T., A. Yeckel, J.J. Derby, X. Luo, M.S. Shephard, E.A. Sander, and V.H. Barocas. Permeability calculations in three-dimensional isotropic and oriented fiber Networks. Physics of Fluids 20(12): 123601. [DOI: 10.1063/1.3021477]

    6.  Stylianopoulos T.,C.A. Bashur, A.S. Goldstein, S.A. Guelcher, and V.H. Barocas. Computational predictions of the tensile properties of electrospun fiber meshes: effect of fiber diameter and fiber orientation. Journal of the Mechanical Behavior of Biomedical Materials 1(4): 326-335. [DOI: 10.1016/j.jmbbm.2008.01.003]

    5.  Chandran P.L., T. Stylianopoulos,and V.H. Barocas. Microstructure based, multiscale modeling for the mechanical behavior of hydrated fiber networks. SIAM Multiscale Modeling and Simulation 7(1): 22-43. [DOI: 10.1137/070689504]

    4.  Stylianopoulos T., A. Aksan, and V.H. Barocas. A structure-based, kinetic model of soft tissue thermomechanics. Biophysical Journal 94(3): 717-725. [DOI: 10.1529/biophysj.107.111716]

    2007

    3.  Stylianopoulos T., and V.H. Barocas. Multiscale structure-based modeling for the elastic mechanical behavior of arterial walls. Journal of Biomechanical Engineering 129(4): 611-618. [DOI: 10.1115/1.2746387]

    2.  Stylianopoulos T., and V.H. Barocas. Volume averaging theory for the study of the mechanics of collagen networks. Computer Methods in Applied Mechanics and Engineering 196(31-32): 2981-2990. [DOI: 10.1016/j.cma.2006.06.019]

    2005

    1.  Oyen M.L., R.F. Cook, T. Stylianopoulos, V.H. Barocas, S.E. Calvin, and D.V. Landers. Uniaxial and biaxial mechanical behavior of human amnion. Journal of Materials Research 20(11): 2902-2909. [DOI: 10.1557/JMR.2005.0382]

    Profile Information

    Diploma in Chemical Engineering, 2003
    National Technical University of Athens, Greece
     
    PhD in Chemical Engineering, 2008
    University of Minnesota, USA
     
    Postdoctoral training, Department of Radiation Oncology
    Massachusetts General Hospital / Harvard Medical School, USA
     

    Triantafyllos received a Diploma in Chemical Engineering from National Technical University of Athens, Greece (GPA: 9.34/10) in 2003. The same year he joined the Chemical Engineering graduate program at the University of Minnesota, USA to pursue his doctoral studies. He worked with Professor Victor Barocas on modeling the mechanical response of native and bioartificial tissues. During his doctoral studies, he was elected twice top doctoral student of the department. After the completion of his PhD., in May 2008, Triantafyllos joined Dr. Rakesh Jain's lab in the department of Radiation Oncology at Harvard Medical School and Massachusetts General Hospital, USA for his prostodoctoral training. In Dr. Jain's lab he performed research in a mixture of experimental and computational problems for optimizing the delivery of molecular and nanoscale medicine to solid tumors. In September 2010, he joined the faculty of the Mechanical and Manufacturing Engineering department at the University of Cyprus.

    Triantafyllos has co-authored over 105 publications in peer-review journals. His publication record includes five articles in Nature journals (Nature Nanotechnology, Nature Medicine, Nature Communications and two articles in Nature Reviews Clinical Oncology), eight articles in PNAS, two in Cancer Research as well as articles in Clinical Cancer Research and ACS Nano. h-index:34, > 8000 citations (Web-of-Science) | h-index:40, > 10600 citations (GoogleScholar).

    He has received 2 highly selective research grant by the European Research Council (ERC Starting Grant ReEngineeringCancer, 2014-2018, 1.44M euros and ERC Consolidator Grant Immuno-Predictor, 2020-2025, 2.0M euros) as well as the prestigious Y. C. Fung Early Carrer award by the American Society of Mechanical Engineers in 2016 (https://www.asme.org/about-asme/participate/honors-awards/achievement-awards/y-c-fung-young-investigator-award). Triantafyllos was also the recipient of the 2014 Most Cited Paper Award by the Biomedical Engineering Society (BMES) of the USA in 2014 and the New Researcher Award by the Research Promotion Foundation of Cyprus the same year.   

    The last 6 years, Triantafyllos has received as a Prinicipal Investigator ~ 5.5 Millions in Research funding, including 7 grants from FP7 and Horizon2020 and 2 grants from Research Promotion Foundation of Cyprus. 

    Triantafyllos is a member of the American Society of Mechanical Engineers, the Biomedical Engineering Society of the USA, the European Foundation of Clinical Nanomedicine, the European Society of Biomechanics and the Greek Association of Computational Mechanics.

    The major focus of my current research is on the biomechanics and drug delivery of solid tumors. I use experimental methods in mice bearing tumors and in vitro tumor models as well as mathematical modelling to study the evolution of mechanical forces in tumors and how these forces correlate with tumors' patho-physiology and delivery of chemotherapy, nanomedicine and immunotherapy. Furthermore, I investigate strategies to optimise tumor blood vessel functionality in order to improve systemic delivery of cancer therapeutics by modifying the mechanical tumor micro-environment.

    Current Funding ID:

    - Research Promotion Foundation of Cyprus, INFRASTRUCTURES/1216/0052, Title: "CancerNanoMED:  Center for Preclinical Evaluation and Optimization of Cancer Nanomedicines".  € 995,812. December 2018 - November 2022.

    - European Research Council, Proof of Concept Grant, (ERC-2018-PoC-838414, CancerFingerPrints), Title: “Identification of nano-mechanical fingerprints as a biomarker for cancer treatment prognosis”. €150,000, May 2019-October 2020.

    - Horizon 2020, Marie Skłodowska-Curie Individual Fellowship, (MSCA-IF-2017-793569 NanoFEITH). Title: "Nanoparticles for fluorescence-enhanced imaging and therapy of breast  cancer ".  €151,649. June  2018 - May 2020.

    - Horizon 2020, Marie Skłodowska-Curie Individual Fellowship, (WF-01-2018-867455 Widening Fellowships, ImmunoMECH), Title: " High-performance biomechanical model of combined immunotherapy and antiangiogenic cancer treatment".  €145, 941. June 2019 - May 2020.

    - Research Promotion Foundation of Cyprus, POST-DOC/0718/0084, Title: “Optimizing immunotherapy in triple-negative breast cancer by normalizing the tumor microenvironment”. €159,924. Expected duration: January 2020-December 2022.

    PAST

    - European Research Council (ERC) Starting Grant (ERC-2013-StG-336839 ReEngineeringCancer).  Title: "Re-engineering the tumor microenvironment to alleviate mechanical stresses and improve chemotherapy".  € 1,440,360. January 2014 - December 2018.   

    - Horizon 2020, Marie Skłodowska-Curie Individual Fellowship, (MSCA-IF-2014-657139 STROMAMECH). Grant Title: "Targeting stromal cells to modify tumor mechanical microenvironment  and optimize drug delivery".  €151,649. May 2015 - April 2017.

     -  Horizon 2020, Marie Skłodowska-Curie Individual Fellowship, (MSCA-IF-2014-658769-MYO-DESMOPLASIA). Grant Title: "Modulating the behaviour of cancer myofibroblasts to control tumour desmoplasia".  €163,649. June 2015 - May 2017.

    -  Young Investigator Award, Research Promotion Foundation of Cyprus, €35,000. June 2015- May 2017.

    -  FP7 Marie-Curie International Reintegration Grant (PIRG08-GA-2010-276894 Cancer Nanomedicine).    Grant Title: "Optimizing the Delivery of Nanomedicine to Solid Tumors". €100,000. August 2011- July  2015.

     (98 articles, h-index=31, >6440 citations Web-of-Science | h-index=36, >8500 citations Google Scholar

     * denotes corresponding authorship

      

    2019

    98. Martin J.D., H. Cabral, T. Stylianopoulos*, and R.K. Jain. Improving cancer immunotherapy using nanomedicine: Progress, opportunities and challenges. Nature Reviews Clinical Oncology (Accepted).

    97. Panagi M. , C. Voutouri, F. Mpekris, P. Papageorgis, M.R. Martin, J.D. Martin, C. Polydorou, M. Kojima, G. Ishii, K. Kataoka and H. Cabral and T.  Stylianopoulos*. TGF-β inhibition combined with cytotoxic nanomedicine normalizes triple negative breast cancer microenvironment towards anti-tumor immunity. Theranostics (Accepted).

    96. Katsamba I., P. Evangelidis and T. Stylianopoulos*. Biomechanical modelling of spinal tumor anisotropic growth. (Under review).

    95. Kalli M., C. Voutouri, A. Minia, V. Pliaka, C. Fotis, L.G. Alexopoulos, and T. Stylianopoulos*. Mechanical Compression inhibits the growth of brain cancer cell spheroids and regulates cell migration through MEK1/Erk1 pathway and GDF15 expression. (Accepted in Frontiers in Oncology).

    94. Gkretsi V., A. Stylianou, M. Kalli, M. Louca, C. Voutouri, and T. Stylianopoulos*.  Growth Differentiation Factor-15 (GDF-15) inhibits cell invasion in breast cancer cells in vitro by suppressing the expression of focal adhesion proteins. (Under review)..

    93. Kontomaris S. V., A. Stylianou, K.S. Nikita, A. Malamou, T. Stylianopoulos*. A simplified approach for the determination of fitting constants in Oliver – Pharr method regarding biological samples. Phys. Biol [DOI: 10.1088/1478-3975/ab252e].

    92. Stylianou A., V. Gkretsi, M. Louca, L.C. Zacharia and T. Stylianopoulos*. Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts. Journal of the Royal Society Interface [DOI: 10.1098/rsif.2019.0226].

    91. Gkretsi V., M. Kalli, C. Efstathiades, P. Papageorgis, V. Papanikolaou, L.C. Zacharia, A. Tsezou, E. Athanassiou, T. Stylianopoulos*, Depletion of Ras Suppressor-1 (RSU-1) promotes cell invasion of breast cancer cells through a compensatory upregulation of a truncated isoform. Scientific Reports [DOI: 10.1038/s41598-019-46575-0]

    90. Louca M., V. Gkretsi, and T. Stylianopoulos*. Coordinated Expression of Ras Suppressor 1 (RSU-1) and Growth Differentiation Factor 15 (GDF15) Affects Glioma Cell Invasion. Cancers  [DOI: 10.3390/cancers11081159].

    89. Martin J.D., M. Panagi, C. Wang, T.T. Khan, M.R. Martin, C. Voutouri, K. Toh, P. Papageorgis, F. Mpekris, C. Polydorou, G. Ishii, S. Takahashi, N. Gotohda, T. Suzuki, M.E. Wilhelm, V.A. Melo, S. Quader, J. Norimatsu, R.M. Lanning, M. Kojima, M.D. Stuber, T. Stylianopoulos, H. Cabral, and K. Kataoka and H. Cabra. The antiemetic dexamethasone increases nanocarrier delivery by normalizing the tumor microenvironment. ACS Nano [DOI: 10.1021/acsnano.8b07865].

    88. Louca M., A. Stylianou, A. Minia, V. Pliaka, L.G. Alexopoulos V. Gkretsi and T. Stylianopoulos*. Ras suppressor-1 (RSU-1) promotes cell invasion in aggressive glioma cells and inhibits it in non-aggressive cells through STAT6 phospho-regulation. Scientific Reports [DOI: 10.1038/s41598-019-44200-8]

    87. Mascheroni P., C.L. Juan, M. Kalli, T. Stylianopoulos, M.H. Michael and H. Hatzikirou. On the impact of chemo-mechanically induced phenotypic transitions in gliomas. Cancers  [DOI: 10.3390/cancers11050716].

    86. Demetriadou C., D. Pavlou, F. Mpekris, C. Achilleos, T. Stylianopoulos, A. Zaravinos, P. Papageorgis and A Kirmizis, Naa40 contributes to colorectal cancer growth by controlling PRMT5 expression. Cell Death & Disease [DOI: 10.1038/s41419-019-1487-3].

    2018

    85. Voutouri C., N.D. Kirkpatrick, E. Chung, F. Mpekris, J.W. Baish, L.L. Munn, D. Fukumura, T. Stylianopoulos* and R.K. Jain. Dynamics of vessel cooption in brain tumors revealed by integrative experimental and mathematical modeling studies. PNAS  [DOI: 10.1073/pnas.1818322116].

    84. Zhao Y., J. Cao, A. Melamed, M. Worley, A. Gockley, D. Jones, H.T. Nia, Y. Zhang, T. Stylianopoulos, A.S. Kumar, F. Mpekris, M. Datta, Y. Sun, L. Wu, X. Gao, O. Yeku, M. del Carmen, D.R. Spriggs, R.K. Jain, and L. Xu. Losartan treatment enhances chemotherapy efficacy and reduces ascites in ovarian cancer models by normalizing the tumor stroma. PNAS [DOI: 10.1073/pnas.1818357116].

    83. Kalli M., F. Mpekris, K.C. Wong, M. Panagi, S. Ozturk, S. Thiagalingam, T. Stylianopoulos and P. Papageorgis. Activin A signaling regulates IL13Rα2 expression to promote breast cancer metastasis. Frontiers in Oncology (Accepted).

    82. Gkretsi, V., Louca, M., Stylianou, A., George Minadakis, G., Spyrou, G.M and Stylianopoulos*, T. Inhibition of breast cancer cell invasion by Ras suppressor-1 (RSU-1) silencing is reversed by Growth Differentiation Factor-15 (GDF-15). Journal of Molecular Sciences, 20 (1) [DOI: 10.3390/ijms20010163].

    81. Kalli M., A. Minia, V. Pliaka, C. Fotis, L.G. Alexopoulos, T. Stylianopoulos*, Solid stress-induced migration is mediated by GDF15 through Akt pathway activation in pancreatic cancer cells. Scientific Reports [DOI: 10.1038/s41598-018-37425-6].

    80. Stylianou A., M. Lekka and T. Stylianopoulos*. AFM Assessing of Nanomechanical FingerPrints for Cancer Early Diagnosis and Classification: from single cell to tissue level. Nanoscale [DOI: 10.1039/C8NR06146G].

    79. Angeli S., K. E. Emblem, P. D. Tonnessen and T. Stylianopoulos*. Towards patient-specific modeling of brain tumor growth and formation of secondary nodes guided by DTI-MRI. NeuroImage: Clinical [DOI: 10.1016/j.nicl.2018.08.032].

    78. Vavourakis V., T. Stylianopoulos, P.A. Wijeratne. In-silico Dynamic Analysis of Cytotoxic Drug Administration to Solid Tumours: Effect of Binding Affinity and Vessel Permeability. PLoS Computational Biology 14(10):e1006460 [DOI: 10.1371/journal.pcbi.1006460].

    77. Kontomaris S.V., A. Stylianou, A. Malamou and T. Stylianopoulos*. A simplified AFM-based model for the calculation of the Young's modulus of cylindrical and spherical shaped samples. Materials Research Express [DOI: 10.1088/2053-1591/aad2c9]

    76. Kitiri E.N., C.K. Varnava, C.S. Patrickios, Voutouri, T. Stylianopoulos, M. Gradzielski and I. Hoffmann. Double-networks Based on Interconnected Amphiphilic “In-out” Star First Polymer Conetworks Prepared by RAFT Polymerization. Journal of Polymer Science, Part A: Polymer Chemistry. [DOI: doi.org/10.1002/pola.29176](Accepted)

    75. Stylianou A., V. Gkretsi, and T. Stylianopoulos*. Atomic Force Microscopy Nano-Characterization of 3D Collagen Gels with Tunable Stiffness. MethodsX  5:503-513 [DOI: 10.1016/j.mex.2018.05.009]

    74. Papaparaskeva G., C. Voutouri, V. Gkretsi, M. Achilleos, T. Stylianopoulos and T. Krasia-Christoforou. Tuning the Mechanical Properties of BIEE-crosslinked Semi-Interpenetrating, Double-Hydrophilic Hydrogels. Macromolecular Materials and Engineering [DOI: 10.1002/mame201700643].

    73. Gkretsi V., and T. Stylianopoulos*. Cell adhesion and matrix stiffness: coordinating cancer cell invasion and metastasis. Frontiers in Oncology  8:145 [DOI: 10.3389/fonc.2018.00145].

    72. Mpekris F., C. Voutouri, P. Papageorgis and T. Stylianopoulos*. Stress alleviation strategy in cancer treatment: Insights from a mathematical model.  Z Angew Math Mech. 2018;1–12 [DOI: 10.1002/zamm.201700270].

    71. Stylianopoulos* T., L.L. Munn, and R.K. Jain. Reengineering the physical microenvironment of tumors to improve drug delivery and efficacy: From math modeling to bench to bedside. Trends in Cancer  4(4):292-319 [DOI: 10.1016/j.trecan.2018.02.005].

    70. Voutouri C. and T. Stylianopoulos*. Accumulation of mechanical forces in tumors is related to hyaluronan content and tissue stiffness. PLoS One 13(3): e0193801 [DOI: 10.1371/journal.pone.0193801].

    69. Kalli M. and T. Stylianopoulos*. Defining the role of solid stress and matrix stiffness in cancer cell proliferation and metastasis. Frontiers in Oncology  8:55 [DOI: 10.3389/fonc.2018.00055].

    68. Stylianou A., V. Gkretsi and T. Stylianopoulos*. Transforming Growth Factor-β modulates Pancreatic Cancer Associated Fibroblasts cell shape, stiffness and invasion. BBA  1862(7):1537-1546 [DOI: 10.1016/j.bbagen.2018.02.009].

    67. Kalli M., P. Papageorgis, V. Gkretsi and T. Stylianopoulos*. Solid stress facilitates fibroblasts activation to promote pancreatic cancer cell migration. Annals of Biomedical Engineering. 46(5):657-669 [DOI: 10.1007/s10439-018-1997-7].

    66. Zacharia LC, T. Stylianopoulos, and V. Gkretsi. Ras Suppressor-1 (RSU-1) in cancer cell metastasis: friend or foe? Critical Reviews in Oncogenesis. 22(3-4):249-253 [DOI: 10.1615/CritRevOncog.2018024231].

    2017

    65. Stylianou A., Gkretsi V., Patrickios C.S., Stylianopoulos* T. Exploring the Nano-Surface of Collagenous and Other Fibrotic Tissues with AFM. In: Rittié L. (eds) Fibrosis. Methods in Molecular Biology, vol 1627. Humana Press, New York, NY [DOI: 0.1007/978-1-4939-7113-8_29]

    64. P.A. Wijeratne, J.H. Hipwell, D.J. Hawkes, T. Stylianopoulos and V. Vavourakis. Multiscale biphasic modelling of peritumoural collagen microstructure: the effect of tumor growth on permeability and fluid flow. PlosOne 12(9): e0184511. [DOI: 10.1371/journal.pone.0184511]

    63. Gkretsi V., L.C. Zacharia, and T. Stylianopoulos*. Targeting inflammation to improve tumor drug delivery. Trends in Cancer 3(9): 621-630. [DOI: 10.1016/j.trecan.2017.07.006]

    62. Mpekris F., P. Papageorgis, C. Polydorou, C. Voutouri, M. Kalli, A.P. Pirentis and T. Stylianopoulos*. Sonic-hedgehog pathway inhibition normalizes desmoplastic tumor microenvironment to improve chemo- and nanotherapy. Journal of Controlled Release 261(10): 105-112. [DOI: 10.1016/j.jconrel.2017.06.022]

    61. Papageorgis P., C. Polydorou, F. Mpekris, C. Voutouri, E. Agathokleous, C. P. Kapnissi-Christodoulou, and T. Stylianopoulos*. Tranilast-induced stress alleviation in solid tumors improves the efficacy of chemo- and nanotherapeutics in a size-independent manner. Scientific Reports 7:46140. [DOI: 10.1038/srep46140]

    60. Gkretsi V., A. Stylianou, M. Louca, and T. Stylianopoulos*. Identification of Ras suppressor-1 (RSU-1) as a potential breast cancer metastasis biomarker using a three-dimensional in vitro approach. Oncotarget 8(16):27364-27379. [DOI: 10.18632/oncotarget.16062]

    59. Angeli S., and T. Stylianopoulos*. Experimental measurements and mathematical modeling towards quantification of brain swelling stress. Journal of Biomechanics 56:42-47. [DOI: 10.1016/j.jbiomech.2017.02.028]

    58. Polydorou C., F. Mpekris, P. Papageorgis, C. Voutouri, and T. Stylianopoulos*. Pirfenidone normalizes the tumor microenvironment to improve chemotherapy. Oncotarget 8(15):24506-24517.[DOI: 10.18632/oncotarget.15534]

    57. Gkretsi V., A. Stylianou, and T. Stylianopoulos*. Vasodilator-Stimulated Phosphoprotein (VASP) depletion from breast cancer MDA-MB-231 cells inhibits tumor spheroid invasion through downregulation of Migfilin, β-catenin and urokinase-plasminogen activator (uPa). Experimental Cell Research 352(2): 281-292. [DOI: 10.1016/j.yexcr.2017.02.019]

    56. Mpekris F., J.W. Baish, T. Stylianopoulos*, and R.K. Jain. Role of vascular normalization in benefit from metronomic chemotherapy. Proceedings of the National Academy of Sciences USA 114(8): 1994-1999. [DOI: 10.1073/pnas.1700340114]

    55. Ambrosi D., S. Pezzuto, D. Riccobelli, T. Stylianopoulos, and P. Ciarletta. Solid tumors are poroelastic solid with chemo-mechanical feedback on growth. Journal of Elasticity 129(1-2):107-124 [DOI: 10.1007/s10659-016-9619-9]

    54. Vavourakis V., P.A. Wijeratne, R. Shipley, M. Loizidou, T. Stylianopoulos, and D.J. Hawkes. A validated multiscale in-silico model for mechano-sensitive tumour angiogenesis and growth. PLoS Computational Biology 13(1): e1005259. [DOI: 10.1371/journal.pcbi.1005259]

    53. Stylianopoulos* T. The solid mechanics of cancer and strategies for improved therapy. Journal of Biomechanical Engineering 139(2): 021004. [DOI: 10.1115/1.4034991]

    52. Kitiri E., C. Patrickios, C. Voutouri, T. Stylianopoulos, I. Hoffmann, R. Schweins, and M. Gradzielski. Synthesis and characterization of double-networks based on pH-responsive, amphiphilic "core-first" star first polymer conetworks prepared by sequential RAFT polymerization. Polymer Chemistry 8(1): 245-259. [DOI: 10.1039/C6PY01340F]

    2016

    51. Alfonso J.C.L., A. Köhn-Luque, T. Stylianopoulos, F. Feuerhake, A. Deutsch, and H. Hatzikirou. Why one-size-fits-all vaso-modulatory interventions fail to control glioma invasion: in silico sights. Scientific Reports 6: 37283. [DOI: 10.1038/srep37283]

    50. Voutouri C., C. Polydorou, P. Papageorgis, V. Gkretsi, and T. Stylianopoulos*. Hyaluronan-derived swelling of solid tumours, the contribution of collagen and cancer cells, and implications for cancer therapy. Neoplasia 18(12): 732-741. [DOI: 10.1016/j.neo.2016.10.001]

    49. Wijeratne P.A., V. Vavourakis, J.H. Hipwell, C. Voutouri, P. Papageorgis, T. Stylianopoulos, A. Evans, and D.J. Hawkes. Multiscale modelling of solid tumour growth: the effect of collagen micromechanics. Biomechanics and Modeling in Mechanobiology 15(5): 1079-1090. [DOI: 10.1007/s10237-015-0745-2]

    48. Achilleos M., F. Mpekris, T. Stylianopoulos, and T. Krasia-Christoforou. Structurally-defined semi-interpenetrating amphiphilic polymer networks with tunable and predictable mechanical response. RSC Advances 6(49): 43278-43283. [DOI: 10.1039/C6RA07376J]

    47. Angeli S., and T. Stylianopoulos*. Biphasic modeling of brain tumor biomechanics and response to radiation treatment. Journal of Biomechanics49(9): 1524-1531. [DOI: 10.1016/j.jbiomech.2016.03.029]

    46. Stylianou A., and T. Stylianopoulos*. Atomic force microscopy probing of cancer cells and tumor microenvironment components. BioNanoScience6(1): 33-46. [DOI: 10.1007/s12668-015-0187-4]

    45. Stylianopoulos* T. Intelligent drug delivery systems for the treatment of solid tumors. European Journal of Nanomedicine 8(1): 9-16. [DOI: 10.1515/ejnm-2015-0041]

    2015

    44. Savva I., E. Evaggelou, G. Papaparaskeva, T. Leontiou, T. Stylianopoulos, F. Mpekris, K. Stylianou, and T. Krasia-Christoforou. Alignment of electrospun polymer fibers using a concave collector. RSC Advances 5(126): 104400-104407. [DOI: 10.1039/C5RA20429A]

    43. Gkretsi V., A. Stylianou, P. Papageorgis, C. Polydorou, and T. Stylianopoulos* T. Remodeling components of the tumor microenvironment to enhance cancer therapy. Frontiers in Oncology 5: 214. [DOI: 10.3389/fonc.2015.00214]

    42. Kepola E.J, E. Loizou, C.S. Patrickios, E. Leontidis, C. Voutouri, T. Stylianopoulos, R. Schweins, M. Gradzielski, C. Krumm, J.C. Tiller, M. Kushnir, and C. Wesdemiotis. Amphiphilic polymer conetworks based on end-linked "core-first" star block copolymers: structure formation with long-range order. ACS Macro Letters 4(9): 1163-1168 (2015) [DOI: 10.1021/acsmacrolett.5b00608]

    41. Stylianopoulos* T., and R.K. Jain. Design considerations for nanotherapeutics in oncology. Nanomedicine: Nanotechnology, Biology and Medicine 11(8): 1893-1907. [DOI: 10.1016/j.nano.2015.07.015]

    40. Papaphilippou P.Ch., I. Vyrides, F. Mpekris, T. Stylianopoulos, C.A. Papatryphonos, C.R. Theocharis, and T. Krasia-Christoforou. Evaluation of novel, cationic electrospun microfibrous membranes as adsorbents in bacteria removal. RSC Advances 5(83): 67617-67629. [DOI: 10.1039/C5RA11406C]

    39. Duda D.G., R.S. Heist, D.V. Sahani, T. Stylianopoulos, J.A. Engelman, and R.K. Jain. Reply to Ciccolini et al.: Using mathematical modeling to predict response to antiangiogenic therapy in cancer patients. Proceedings of the National Academy of Sciences USA 112(27): E3454. [DOI: 10.1073/pnas.1507225112]

    38. Mpekris F., S. Angeli, A.P. Pirentis, and T. Stylianopoulos*. Stress-mediated progression of solid tumors: effect of mechanical stress on tissue oxygenation, cancer cell proliferation and drug delivery. Biomechanics and Modeling in Mechanobiology 14(6): 1391-1402. [DOI: 10.1007/s10237-015-0682-0]

    37. Pirentis A.P., C. Polydorou, P. Papageorgis, C. Voutouri, F. Mpekris, and T. Stylianopoulos*. Remodelling of extracellular matrix due to solid stress accumulation during tumour growth. Connective Tissue Research 56(5): 345-354. [DOI: 10.3109/03008207.2015.1047929]

    36. Stylianopoulos* T., E.-A. Economides, J.W. Baish, D. Fukumura, and R.K. Jain. Towards optimal design of cancer nanomedicines: multi-stage nanoparticles for the treatment of solid tumors. Annals of Biomedical Engineering 43(9): 2291-2300. [DOI: 10.1007/s10439-015-1276-9]

    35. Mpekris F., M. Achilleos, E. Vasile, E. Vasile, T. Krasia-Christoforou, and T. Stylianopoulos*. Mechanical properties of structurally-defined magnetoactive polymer (co)networks. RSC Advances 5(26): 20011-20019. [DOI: 10.1039/C4RA16260A]

    34. Papageorgis P., and T. Stylianopoulos*. Role of TGFβ in regulation of the tumor microenvironment and drug delivery. International Journal of Oncology 46(3): 933-943. [DOI: 10.3892/ijo.2015.2816]

    2014

    33. Voutouri C., and T. Stylianopoulos*. Evolution of osmotic pressure in solid tumors. Journal of Biomechanics 47(14): 3441-3447. [DOI:10.1016/j.jbiomech.2014.09.019]

    32. Voutouri C., F. Mpekris, P. Papageorgis, A.D. Odysseos, and T. Stylianopoulos*. The role of constitutive behavior and tumor-host mechanical interactions in the state of stress and growth of solid tumors. PLoS One 9(8): e104717. [DOI: 10.1371/journal.pone.0104717]

    31. Chauhan V.P., Y. Boucher, C.R. Ferrone, S. Roberge, J.D. Martin, T. Stylianopoulos, N. Bardeesy, R.A. DePinho, T.P. Padera, L.L. Munn, and R.K. Jain. Compression of pancreatic tumor blood vessels by hyaluronan is caused by solid stress and not interstitial fluid pressure. Cancer Cell 26(1): 14-15. [DOI: 10.1016/j.ccr.2014.06.003]

    30. Jain R.K., J.D. Martin, and T. Stylianopoulos*. The role of mechanical forces in tumor progression and therapy. Annual Reviews of Biomedical Engineering 16: 321-346. [DOI: 10.1146/annurev-bioeng-071813-105259]

    29. Achilleos A., C. Loizidis, M. Hadjiandreou, T. Stylianopoulos, and G. Mitsis. Multiprocessing linear dynamics modeling for personalized tumor growth prediction. Annals of Biomedical Engineering 42(5): 1095-1111. [DOI: 10.1007/s10439-014-0975-y]

    2013

    28. Stylianopoulos* T., and R.K. Jain. Combining two strategies to improve perfusion and drug delivery in solid tumors. Proceedings of the National Academy of Sciences USA 110(46): 18632-18637. [DOI: 10.1073/pnas.1318415110]

    27. Huang Y., T. Stylianopoulos, D. Duda, D. Fukumura, and R.K. Jain. Benefits of vascular normalization are dose and time dependent. Cancer Research 73(23): 7144-7146. [DOI: 10.1158/0008-5472.CAN-13-1989]

    26. Chauhan V.P., J.D. Martin, H. Liu, D.A. Lacorre, S.R. Jain, S.V. Kozin, T. Stylianopoulos, A.S. Mousa, X. Han, P. Adstamongkonkul, Z. Popovic, P. Huang, M.G. Bawendi, Y. Boucher and R.K. Jain. Angiotensin inhibition enhances drug delivery and potentiates chemotherapy by decompressing tumor blood vessels. Nature Communications 4: 2516. [DOI: 10.1038/ncomms3516]

    25. Stylianopoulos* T., J.D. Martin, M. Snuderl, F. Mpekris, S. Jain, and R.K. Jain. Coevolution of solid stress and interstitial fluid pressure in tumor during progression: Implications for vascular collapse. Cancer Research 73(13): 3833-3841. [DOI: 10.1158/0008-5472.CAN-12-4521]

    24. Stylianopoulos* T. EPR-effect: utilizing size-dependent nanoparticle delivery to solid tumors. Therapeutic delivery 4.4: 421-423. [DOI: 10.4155/tde.13.8]

    23. Stylianopoulos* T., K. Soteriou, D. Fukumura, and R.K. Jain. Cationic nanoparticles have superior transvascular flux in solid tumors: Insights from a mathematical model. Annals of Biomedical Engineering 41(1): 68-77. [DOI: 10.1007/s10439-012-0630-4]

      

    2012

    22. Stylianopoulos* T., M. Kokonou, S. Michael, A. Tryfonos, C. Rebholz, A.D. Odysseos, and D. Doumanidis. Tensile mechanical properties and hydraulic permeabilities of electrospun cellulose acetate fiber meshes. Journal of Biomedical Materials Research - Part B Applied Biomaterials 100(8): 2222-2230. [DOI: 10.1002/jbm.b.32791]

    21. Stylianopoulos T., J.D. Martin, V.P. Chauhan, S.R. Jain, B. Diop-Frimpong, B. Smith, C.R. Ferrone, F. Hornicek, Y. Boucher, L.L. Munn, and R.K. Jain. Causes, consequences, and remedies for growth-induced solid stress in murine and human tumors. Proceedings of the National Academy of Sciences USA 109(38): 15101-15108. [DOI: 10.1073/pnas.1213353109]

    20. Chauhan V.P., T. Stylianopoulos, J.D. Martin, Z. Popovic, W.S. Kamoun, M.G. Bawendi, D. Fukumura, and R.K. Jain. Normalization of tumour blood vessels improves the delivery of nanomedicines in a size-dependent manner. Nature Nanotechnology 7: 383-388. [DOI: 10.1038/nnano.2012.45]

    19. Stylianopoulos T., C. Wong, M.G. Bawendi, R.K. Jain, and D. Fukumura. Multistage nanoparticles for improved delivery into tumor tissue. Methods in Enzymology 508: 109-130. [DOI: 10.1016/B978-0-12-391860-4.00006-9]

    PI's pre-CBL publications

    2011

    18. Chauhan V.P., T. Stylianopoulos, Y. Boucher, and R.K. Jain. Delivering of molecular and nanoscale medicine to tumors: transport barriers and strategies. Annual Reviews of Chemical and Biomolecular Engineering 2: 281-298. [DOI: 10.1146/annurev-chembioeng-061010-114300]

    17. Wong C.R., T. Stylianopoulos, J. Cui, J. Martin, V.P. Chauhan, W. Jiang, Z. Popovic, R.K. Jain, M.G. Bawendi, and D. Fukumura. Multistage nanoparticle delivery system for deep penetration into tumor tissue. Proceedings of the National Academy of Sciences USA 108(6): 2426-2431. [DOI: 10.1073/pnas.1018382108]

    16. Baish J.W., T. Stylianopoulos, R.M. Lanning, W. Kamoun, L.L. Munn, D. Fukumura, and R.K. Jain. Scaling rules for diffusive drug delivery in tumor and normal tissues. Proceedings of the National Academy of Sciences USA 108(5): 1799-1803. [DOI: 10.1073/pnas.1018154108]

    2010

    15. Stylianopoulos T., B. Diop-Frimpong, L.L. Munn, and R.K. Jain. Diffusion anisotropy in collagen gels and tumors: the effect of fiber network orientation. Biophysical Journal 99(10): 3119-3128. [DOI: 10.1016/j.bpj.2010.08.065]

    14. Jain R.K., and T. Stylianopoulos. Delivering nanomedicine to solid tumors. Nature Reviews Clinical Oncology 7(11): 653-664. [DOI: 10.1038/nrclinonc.2010.139]

    13. Stylianopoulos T., M.Z. Poh, N. Insin, L.L. Munn, D. Fukumura, M. Bawendi, and R.K. Jain. Diffusion of particles in the extracellular matrix: effect of repulsive electrostatic interactions. Biophysical Journal 99(5): 1342-1349. [DOI: 10.1016/j.bpj.2010.06.016]

    2009

    12. Vakoc V.J., R.M. Lanning, J.A. Tyrrell, T.P. Padera, L. Bartlett, T. Stylianopoulos, L.L. Munn, G.J. Tearney, D. Fukumura, R.K. Jain, and B.E. Bouma. Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging. Nature Medicine 15(10): 1219-1223. [DOI: 10.1038/nm.1971]

    11. Mok W., T. Stylianopoulos, Y. Boucher, and R.K. Jain. Mathematical modeling of herpes simplex virus distribution in solid tumors: implications for cancer gene therapy. Clinical Cancer Research 15(7): 2352-2360. [DOI: 10.1158/1078-0432.CCR-08-2082]

    10. Sander E.A., T. Stylianopoulos, R.T. Tranquillo, and V.H. Barocas. Image-based multi-scale modeling predicts tissue-level and network-level fiber reorganization in stretched cell-compacted collagen gels. Proceedings of the National Academy of Sciences USA 106(42): 17675-17680. [DOI: 10.1073/pnas.0903716106]

    9.  Sander E.A., T. Stylianopoulos, R.T. Tranquillo, and V.H. Barocas. Image-based biomechanics of collagen-based tissue equivalents. IEEE Engineering in Medicine and Biology 28(3): 10-18. [DOI: 10.1109/MEMB.2009.932486]

    8.  Luo X., T. Stylianopoulos,V.H. Barocas, and M.S. Shephard. Multiscale computation for bioartificial soft tissues with complex geometries. Journal of Engineering with Computers 25(1): 87-95. [DOI: 10.1007/s00366-008-0111-4]

    2008

    7.  Stylianopoulos T., A. Yeckel, J.J. Derby, X. Luo, M.S. Shephard, E.A. Sander, and V.H. Barocas. Permeability calculations in three-dimensional isotropic and oriented fiber Networks. Physics of Fluids 20(12): 123601. [DOI: 10.1063/1.3021477]

    6.  Stylianopoulos T.,C.A. Bashur, A.S. Goldstein, S.A. Guelcher, and V.H. Barocas. Computational predictions of the tensile properties of electrospun fiber meshes: effect of fiber diameter and fiber orientation. Journal of the Mechanical Behavior of Biomedical Materials 1(4): 326-335. [DOI: 10.1016/j.jmbbm.2008.01.003]

    5.  Chandran P.L., T. Stylianopoulos,and V.H. Barocas. Microstructure based, multiscale modeling for the mechanical behavior of hydrated fiber networks. SIAM Multiscale Modeling and Simulation 7(1): 22-43. [DOI: 10.1137/070689504]

    4.  Stylianopoulos T., A. Aksan, and V.H. Barocas. A structure-based, kinetic model of soft tissue thermomechanics. Biophysical Journal 94(3): 717-725. [DOI: 10.1529/biophysj.107.111716]

    2007

    3.  Stylianopoulos T., and V.H. Barocas. Multiscale structure-based modeling for the elastic mechanical behavior of arterial walls. Journal of Biomechanical Engineering 129(4): 611-618. [DOI: 10.1115/1.2746387]

    2.  Stylianopoulos T., and V.H. Barocas. Volume averaging theory for the study of the mechanics of collagen networks. Computer Methods in Applied Mechanics and Engineering 196(31-32): 2981-2990. [DOI: 10.1016/j.cma.2006.06.019]

    2005

    1.  Oyen M.L., R.F. Cook, T. Stylianopoulos, V.H. Barocas, S.E. Calvin, and D.V. Landers. Uniaxial and biaxial mechanical behavior of human amnion. Journal of Materials Research 20(11): 2902-2909. [DOI: 10.1557/JMR.2005.0382]
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