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ΧΑΡΑΛΑΜΠΟΥΣ ΑΝΝΑ
CHARALAMBOUS ANNA
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ΕΙΔΙΚΟ ΕΚΠΑΙΔΕΥΤΙΚΟ ΠΡΟΣΩΠΙΚΟ
Τμήμα Βιολογικών Επιστημών
ΘΕΕ 01 - Σχολή Θετικών και Εφαρμοσμένων Επιστημών
Πανεπιστημιούπολη
B133
22893971
22895095
-

Προσωπικό Προφίλ

ΕΚΠΑΙΔΕΥΣΗ

2001-2006       PhD, The Rockefeller University. Improved immunity to tumor antigens following delivery to maturing dendritic cells in mice (advisor: Professor Ralph Steinman, Nobel Laureate)

1997-2001       ΒSc. Molecular Biochemistry and Biophysics, Yale University. Cum Laude and Highest Honors 

Τα ερευνητικά μου ενδιαφέροντα εστιάζονται σε θέματα κυτταρικής και μοριακής ανοσολογίας, καθώς επίσης και στην ανάπτυξη αντικαρκινικών θεραπειών, που είτε βασίζονται στην επάνδρωση δενδριτικών κυττάρων για  επαγωγή ογκο-ειδικής κυτταρικής ανοσίας, είτε στοχεύουν συγκεκριμένους μοριακούς/ κυτταρικούς μηχανισμούς για αναστολή της μετάστασης. Επιπλέον, στα ενδιαφέροντα αυτά εντάσσονται θέματα που αφορούν την ανάπτυξη μεθόδων βιοαπεικόνισης και νανοτεχνολογίας με πιθανές εφαρμογές στην μελέτη του καρκίνου.

 Peer Reviewed Original Articles
  1. Antoniades, I., Kyriakou, M., Charalambous A., Kalalidou, K. and Skourides, P. “FAK displacement from Focal Adhesions: A promising new strategy for the generation of FAK inhibitors to target cancer progression and metastasis”, accepted with minor revisions by JBC
  2. Charalambous A*, Antoniades, I*., Christodoulou, N., Zanardelli, S. and Skourides P “Determining the temporal and spatial expression of Calpains in amphibians”, accepted in Methods in Molecular Biology in May 2018, *Equal Contribution
  3. Antoniades, I*., Charalambous A*, Christodoulou, N., Zanardelli, S. and Skourides P “Determining the role of Calpains in embryo development in amphibians”, accepted in Methods in Molecular Biology in May 2018 *Equal Contribution
  4. Charalambous A, Koyioni M, Antoniades I, Pegeioti D, Eleftheriou I, Michaelidou S, Amelichev A, Konstantinova L, Rakitin O, Koutentis P* and Skourides P.* “1,2,3-Dithiazoles-new reversible melanin synthesis inhibitors: a chemical genomics study”, Chem. Commun., 2015,6, 935-946
  5. Ioannou, A., Eleftheriou, I., Charalambous, A.,Skourides, P.A. “Single and multiplexed fluorescent in-situ hybridization using quantum dots nanocrystals in Xenopus” (2015) Neuromethods 99, 451-462
  6. Charalambous A, Andreou M., Antoniades, I., Christodoulou, N. and Skourides P. “In vivo, site-specific covalent conjugation of Quantum Dots to proteins via split-intein splicing” (2012) Methods in Molecular Biology 906, p. 157-169
  7. Ioannou, A., Eleftheriou, I., Lubatti, A., Charalambous, A.,Skourides, P.A. “High-resolution whole mount in-situ hybridization using quantum dot nanocrystals” (2012) Journal of Biomedicine and Biotechnology  2012                                
  8. Charalambous A*, Antoniades, I*., Christodoulou, N. and Skourides P. “Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivoJournal of Nanobiotechnology 2011, 9:37 (15 September 2011) *Equal Contribution
  9. Charalambous A, Andreou M. and Skourides P. “Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivoJournal of Nanobiotechnology 2009, 7:9 (10 December 2009)
  10. Wang, B., Kuroiwa, J., He, L., Charalambous, A., Keler, T., Steinman, RM. The human cancer antigen mesothelin is more efficiently presented to the mouse immune system when targeted to the DEC205 receptor on Dendritic Cells. Annals of the NYAS, 2009, Volume 1174 Issue Cancer Vaccines Sixth International Symposium, Pages 6-17
  1. Radek S.*, Charalambous A.*, Mazumder A., Vesole D., Jagannath S. Dhodapkar M. Bortezomib enhances dendritic cell (DC) mediated induction of immunity to human myeloma via exposure of cell surface heat shock protein 90 on dying tumor cells: Therapeutic implications. E-pub 2007 Feb 13. *Equal Contribution                        
  2. Charalambous A., Oks M., Nchinda G., Yamazaki S., Steinman RM. Dendritic Cell targeting of survivin protein in a xenogeneic form elicits CD4+ T cell immunity to mouse survivin. J Immunol. 2006 Dec 15;177(12):8410-21
  3. Liu K., Idoyaga J., Charalambous A., Fujii S., Bonito A., Oks M., Mordoh J., Wainstok R., Bai XF., Liu Y., Steinman RM. Innate NKT Lymphocytes Confer Superior Adaptive Immunity Via Tumor Capturing Dendritic Cells. J Exp Med. 2006 202(11):1507-116.           
  4. Bonifaz LC, Bonnyay DP, Charalambous A, Darguste DI, Fujii S, Soares H, Brimnes MK, Moltedo B, Moran TM, Steinman RM. In Vivo Targeting of Antigens to Maturing Dendritic Cells via the DEC-205 Receptor Improves T Cell Vaccination. J Exp Med. 2004 Mar 15;199(6):815-24.                                        
  5. Kang YS, Kim JY, Bruening SA, Pack M, Charalambous A, Pritsker A, Moran TM, Loeffler JM, Steinman RM, Park CG. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. Proc Natl Acad Sci U S A. 2004; 101(1):215-20. Epub 2003 Dec 23.
  6. Hall D, Dhilla A, Charalambous A, Gogos JA, Karayiorgou M. Sequence variants of the brain-derived neurotrophic factor (BDNF) gene are strongly associated with obsessive-compulsive disorder. Am J Hum Genet. 2003 Aug;73(2):370-6. Epub 2003 Jun 27.

Book Contributions

  1. Ioannou, A., Eleftheriou, I., Charalambous, A.,Skourides, P.A. “Single and multiplexed fluorescent in-situ hybridization using quantum dots nanocrystals in Xenopus” (2015) In situ Hybridization Methods, p.451-462
  2. Charalambous, A., Andreou, M., Antoniades, I., Christodoulou, N., Skourides, P.A. "In vivo site-specific, covalent conjugation of quantum dots to proteins via split-intein splicing" (2012) Methods in Molecular Biology906, p. 157 - 169   
  3. Liu K., Charalambous A., Steinman R. Some biological features of dendritic cells in mouse. In The Mouse in Biomedical Research, Volume 3: Normative Biology, Immunology and Husbandry, 2nd Ed, Elsevier, San Diego, eds JG Fox, C Newcomer, A Smith, S. Barthold, F Quimby, and M Davisson. 2006

Profile Information

EDUCATION

2001-2006       PhD, The Rockefeller University. Improved immunity to tumor antigens following delivery to maturing dendritic cells in mice (advisor: Professor Ralph Steinman, Nobel Laureate)

1997-2001       ΒSc. Molecular Biochemistry and Biophysics, Yale University. Cum Laude and Highest Honors 

My research interests are focused on cell and molecular immunology, as well as the development of therapeutic or preventive cancer vaccines based on either the recruitment of Dendritic Cells to elicit tumor antigen-specific immune responses or based on targeting specific molecular/cellular mechanisms to inhibit metastasis. In addition, I am also interested in the development of bioimaging techniques and nanotechnology that could have applications in cancer research. 
 Peer Reviewed Original Articles
  1. Antoniades, I., Kyriakou, M., Charalambous A., Kalalidou, K. and Skourides, P. “FAK displacement from Focal Adhesions: A promising new strategy for the generation of FAK inhibitors to target cancer progression and metastasis”, accepted with minor revisions by JBC
  2. Charalambous A*, Antoniades, I*., Christodoulou, N., Zanardelli, S. and Skourides P “Determining the temporal and spatial expression of Calpains in amphibians”, accepted in Methods in Molecular Biology in May 2018, *Equal Contribution
  3. Antoniades, I*., Charalambous A*, Christodoulou, N., Zanardelli, S. and Skourides P “Determining the role of Calpains in embryo development in amphibians”, accepted in Methods in Molecular Biology in May 2018 *Equal Contribution
  4. Charalambous A, Koyioni M, Antoniades I, Pegeioti D, Eleftheriou I, Michaelidou S, Amelichev A, Konstantinova L, Rakitin O, Koutentis P* and Skourides P.* “1,2,3-Dithiazoles-new reversible melanin synthesis inhibitors: a chemical genomics study”, Chem. Commun., 2015,6, 935-946
  5. Ioannou, A., Eleftheriou, I., Charalambous, A.,Skourides, P.A. “Single and multiplexed fluorescent in-situ hybridization using quantum dots nanocrystals in Xenopus” (2015) Neuromethods 99, 451-462
  6. Charalambous A, Andreou M., Antoniades, I., Christodoulou, N. and Skourides P. “In vivo, site-specific covalent conjugation of Quantum Dots to proteins via split-intein splicing” (2012) Methods in Molecular Biology 906, p. 157-169
  7. Ioannou, A., Eleftheriou, I., Lubatti, A., Charalambous, A.,Skourides, P.A. “High-resolution whole mount in-situ hybridization using quantum dot nanocrystals” (2012) Journal of Biomedicine and Biotechnology  2012                                
  8. Charalambous A*, Antoniades, I*., Christodoulou, N. and Skourides P. “Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivoJournal of Nanobiotechnology 2011, 9:37 (15 September 2011) *Equal Contribution
  9. Charalambous A, Andreou M. and Skourides P. “Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivoJournal of Nanobiotechnology 2009, 7:9 (10 December 2009)
  10. Wang, B., Kuroiwa, J., He, L., Charalambous, A., Keler, T., Steinman, RM. The human cancer antigen mesothelin is more efficiently presented to the mouse immune system when targeted to the DEC205 receptor on Dendritic Cells. Annals of the NYAS, 2009, Volume 1174 Issue Cancer Vaccines Sixth International Symposium, Pages 6-17
  1. Radek S.*, Charalambous A.*, Mazumder A., Vesole D., Jagannath S. Dhodapkar M. Bortezomib enhances dendritic cell (DC) mediated induction of immunity to human myeloma via exposure of cell surface heat shock protein 90 on dying tumor cells: Therapeutic implications. E-pub 2007 Feb 13. *Equal Contribution                        
  2. Charalambous A., Oks M., Nchinda G., Yamazaki S., Steinman RM. Dendritic Cell targeting of survivin protein in a xenogeneic form elicits CD4+ T cell immunity to mouse survivin. J Immunol. 2006 Dec 15;177(12):8410-21
  3. Liu K., Idoyaga J., Charalambous A., Fujii S., Bonito A., Oks M., Mordoh J., Wainstok R., Bai XF., Liu Y., Steinman RM. Innate NKT Lymphocytes Confer Superior Adaptive Immunity Via Tumor Capturing Dendritic Cells. J Exp Med. 2006 202(11):1507-116.           
  4. Bonifaz LC, Bonnyay DP, Charalambous A, Darguste DI, Fujii S, Soares H, Brimnes MK, Moltedo B, Moran TM, Steinman RM. In Vivo Targeting of Antigens to Maturing Dendritic Cells via the DEC-205 Receptor Improves T Cell Vaccination. J Exp Med. 2004 Mar 15;199(6):815-24.                                        
  5. Kang YS, Kim JY, Bruening SA, Pack M, Charalambous A, Pritsker A, Moran TM, Loeffler JM, Steinman RM, Park CG. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. Proc Natl Acad Sci U S A. 2004; 101(1):215-20. Epub 2003 Dec 23.
  6. Hall D, Dhilla A, Charalambous A, Gogos JA, Karayiorgou M. Sequence variants of the brain-derived neurotrophic factor (BDNF) gene are strongly associated with obsessive-compulsive disorder. Am J Hum Genet. 2003 Aug;73(2):370-6. Epub 2003 Jun 27.

Book Contributions

  1. Ioannou, A., Eleftheriou, I., Charalambous, A.,Skourides, P.A. “Single and multiplexed fluorescent in-situ hybridization using quantum dots nanocrystals in Xenopus” (2015) In situ Hybridization Methods, p.451-462
  2. Charalambous, A., Andreou, M., Antoniades, I., Christodoulou, N., Skourides, P.A. "In vivo site-specific, covalent conjugation of quantum dots to proteins via split-intein splicing" (2012) Methods in Molecular Biology906, p. 157 - 169   
  3. Liu K., Charalambous A., Steinman R. Some biological features of dendritic cells in mouse. In The Mouse in Biomedical Research, Volume 3: Normative Biology, Immunology and Husbandry, 2nd Ed, Elsevier, San Diego, eds JG Fox, C Newcomer, A Smith, S. Barthold, F Quimby, and M Davisson. 2006